Molecular Genetics of Salt Tolerance in Tomato F2 Segregating Population with the Aid of RAPD Markers

Authors: Vincent Ezin, Tatsiana Dasenka, Pelagie Bidossessi Agbobatinkpo,
Adam Ahanchede, Avtar Krishan Handa

Salt tolerance of segregating progenies of a cross between a domesticated salt sensitive tomato cultivar (CA4) and a natural salt-tolerant wild-type tomato species (LA1606) was characterized. The F1 plants from this cross were selfed and 120 F2 segregating progenies from the resulting population along with parental CA4 and LA1606 plants were evaluated for salt tolerance. These plants were irrigated everyday with 185 mM NaCl for 82 days and quantitative traits were quantified including number of flowers, fruit number, fruit weight, fruit length, fruit width, fruit set percentage, and total yield. The two parental lines were evaluated for the presence of 27 seven independent RAPD markers and 7 markers were found to be polymorphic for the two genotypes. Bulk Segregant (BSA) analyses consisting of pooling 10 “most tolerant” and 10 “most sensitive” F2 segregating plants showed association of two RAPD polymorphic markers with higher salt tolerance. Two DNA markers that exhibit co-segregation with salt tolerance were identified and characterized. RAPD marker OPX-17 and MRTOMR-022 exhibited 2 positive molecule markers (polymorphism) which were found only in the resistant parent (LA1606) and resistant F2 bulk.


Journal: Agricultural Sciences
DOI: as.2018.912109(PDF)
Paper Id: 89052 (metadata)

See also: Comments to Paper

About scirp

(SCIRP: is an academic publisher of open access journals. It also publishes academic books and conference proceedings. SCIRP currently has more than 200 open access journals in the areas of science, technology and medicine. Readers can download papers for free and enjoy reuse rights based on a Creative Commons license. Authors hold copyright with no restrictions. SCIRP calculates different metrics on article and journal level. Citations of published papers are shown based on Google Scholar and CrossRef. Most of our journals have been indexed by several world class databases. All papers are archived by PORTICO to guarantee their availability for centuries to come.
This entry was posted in AS. Bookmark the permalink.

Leave a Reply

Your email address will not be published. Required fields are marked *