Authors: Raquel de Oliveira Marreiro, Maria Fulgência Costa Lima Bandeira, Carina Toda, Fábio Correia Sampaio, Tatiane Pereira de Souza, Gisely Naura Venâncio,
Wanderleia Monteiro de Souza, Nikeila Chacon de Oliveira Conde
This study aimed to evaluate the antimicrobial activity of the extract of Libidibia ferrea L. (228.022-INPA) and a mouthwash formulation against microorganisms of the dental biofilm. The extract of Libidibia ferrea L. was prepared and formulated with an alcohol-free mouthwash. The Minimum Inhibitory Concentration—MIC was determined by microdilution and cell viability was evaluated through fluorescence. The MICs for the extract were 4.375 μg/mL (Streptococcus mutans), 3.750 μg/mL (Streptococcus oralis), 4.375 μg/mL (Lactobacillus casei). In contrast, for the mouthwash, the MICs were 6.000 μg/mL (bacteriostatic against Streptococcus mutans and bactericidal against Streptococcus oralis). In 1.250 μg/mL of the extract, the number of viable cells measured by fluorescence tests was 16.47% and 14.12% for Streptococcus mutans and Lactobacillus casei, respectively. For the mouthwash (1.000 μg/mL), 20.60% of viable cells for Streptococcus mutans, and 9.60% for Lactobacillus casei were observed. It can be concluded that the extract and its mouthwash containig Libidibia ferrea L. showed antibacterial activity against microorganisms from the dental biofilm.
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